Gibberellins in immature seeds and dark-grown shoots of Pisum sativum |
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Authors: | Paul Gaskin Sarah J Gilmour Jake MacMillan Valerie M Sponsel |
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Institution: | (1) Agricultural and Food Research Council Research Group, School of Chemistry, University of Bristol, BS8 1TS Bristol, UK |
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Abstract: | Gibberellins (GAs) A17, A19, A20, A29, A44, 2OH-GA44 (tentative) and GA29-catabolite were identified in 21-day-old seeds of Pisum sativum cv. Alaska (tall). These GAs are qualitatively similar to those in the dwarf cultivar Progress No. 9 with the exception of GA19 which does not accumulate in Progress seeds. There was no evidence for the presence of 3-hydroxylated GAs in 21 day-old Alaska seeds. Dark-grown shoots of the cultivar Alaska contein GA1, GA8, GA20, GA29, GA8-catabolite and GA29-catabolite. Dark-grown shoots of the cultivar Progress No.9 contain GA8, GA20, GA29 and GA29-catabolite, and the presence of GA1 was strongly indicated. Quantitation using GAs labelled with stable isotope showed the level of GA1 in dark-grown shoots of the two cultivars to be almost identical, whilst the levels of GA20, GA29 and GA29-catabolite were significantly lower in Alaska than in Progress No. 9. The levels of these GAs in dark-grown shoots were 102- to 103-fold less than the levels in developing seeds. The 2-epimer of GA29 is present in dark-grown-shoot extracts of both cultivars and is not thought to be an artefact.Abbreviations cv
cultivar
- GAn
gibberellin An
- GC
gas chromatography
- GC-MS
combined gas chromatographymass spectrometry
- HPLC
high-pressure liquid chromatography
- KRI
Kovats retention index
- MeTMSi
methyl ester trimethylsilyl ether |
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Keywords: | Gibberellin (identification quantitation) Pisum (tall and dwarf cultivars) Seed developing (gibberellin) Shoot dark grown (gibberellin) |
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