Characterization of gibberellins from light-grown Phaseolus coccineus seedlings by combined GC-MS |
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Authors: | DH Bowen A Crozier J MacMillan DM Reid |
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Institution: | School of Chemistry, University of Bristol, Bristol, England;Botany Department, University of Canterbury, Christchurch, New Zealand;School of Chemistry, University of Bristol, Bristol, England;Biology Department, University of Calgary, Calgary, Alberta, Canada |
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Abstract: | Following extensive purification of an extract from 5000 light-grown Phaseolus coccineus seedlings by procedures including countercurrent distribution, Sephadex G10, polyvinylpyrrolidone, charcoal-celite and silicic acid partition column chromatography, TLC preparative GLC, gibberellins A1, A4, A5 and A20 were characterized by combined GC-MS. In addition, an unknown compound isomeric with gibberellin A17 but lacking an hydroxyl group, was also detected. |
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Keywords: | Leguminosae combined GC-MS |
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