Multiple forms of peroxidase from Narcissus pseudonarcissus |
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Authors: | Alfred F Renaldo David T Bailey Glenn M Nagel |
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Institution: | Department of Chemistry and Institute for Molecular Biology, California State University, Fullerton, CA 92634, U.S.A. |
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Abstract: | Multiple forms of peroxidase from Narcissus pseudonarcissus were identified and separated by polyacrylamide gel electrophoresis. The enzyme forms were found to be particulate but could be solubilized in buffers of high ionic strength and high pH. Bulbs at different stages (dormant, early growth, flowering and post-flowering) were investigated and both the number and distribution of peroxidase forms were found to differ. The major peroxidase form in dormant bulbs was purified and displayed a number of notable properties including a MW of at least 105, a high isoelectric point and the apparent absence of a heme prosthetic group. |
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Keywords: | Amaryllidaceae King Alfred daffodil identification isolation enzyme peroxidase |
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