Partial structure and properties of the ferredoxin from Rhodymenia palmata |
| |
Authors: | PW Andrew LJ Rogers BG Haslett D Boulter |
| |
Institution: | 1. Department of Biochemistry, University College of Wales, Aberystwyth, SY23 3DD, U.K.;2. Department of Botany, University of Durham, Durham, DH1 3LE, U.K. |
| |
Abstract: | A ferredoxin of MW 11 000 was isolated from the marine alga Rhodymenia palmata (Palmaria palmata). In its oxidised form the ferredoxin had absorption maxima at 276, sh 281, 328, 423 and 465 nm, and contained a single 2Fe-2S] cluster. The midpoint potential of the ferredoxin was ?400 mV and it effectively mediated electron transport in NADP+-photoreduction by higher plant chloroplasts, and pyruvate decarboxylation by the phosphoroclastic system of an anacrobic bacterium. The amino acid composition was Lys3, His1, Arg1, Asx12, Thr9, Ser8, Glx13, Pro4, Gly8, Ala7, Cys5, Val8, Ile4, Leu9, Tyr4, Phe2; tryptophan and methionine were absent from the molecule. The N-terminal amino acid region consisting of ca half the total amino acid sequence was determined using an automatic sequencer. |
| |
Keywords: | Rhodophyta red algae ferredoxin amino acid sequence |
本文献已被 ScienceDirect 等数据库收录! |
|