Direct PCR of washed blood cells. |
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Authors: | B Y Nordv?g G Husby M Raafat el-Gewely |
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Institution: | Department of Rheumatology, Institute of Clinical Medicine, University Hospital of Troms?, Norway. |
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Abstract: | We report a simple and rapid method for direct DNA amplification of washed blood cells by PCR. Small samples (2-100 microliters) of blood were washed, the cells resuspended in a buffer and used directly for PCR after boiling. Amplification of a specific DNA sequence of the human transthyretin gene, directed by the primers, was successfully performed. The method gives comparable results to amplifications made by purified DNA from blood. |
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