DNA content analysis of insect cell lines by flow cytometry |
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Authors: | Xavier Léry Guy Charpentier Serge Belloncik |
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Institution: | (1) Entomovirology laboratory, ORSTOM, B.O. 26, Giza code 12211 Cairo, Egypt and;(2) Département de Chimie-Biologie, Université du Québec à Trois-Rivières, Trois-Rivières (, Qc, G9A 5H7, Canada;(3) Virology Research Center, Institut Armand-Frappier, 531 Boulevard des Prairies, Laval-des-Rapides, H7N 4Z3, Canada |
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Abstract: | The DNA content of insect cell lines (6 lepidoptera, 1 coleoptera and 1 diptera) was determined by flow cytometry. The DNA
profiles of the 8 cell lines tested were different. They were characterized by the presence of several peaks (2 to 7) corresponding
to different ploidy levels, by differences in the fluorescence intensity of each peak and by the proportion of cells in each
peak. Two cell lines (Cf124 and BmN) were constituted of 2 distinct populations of cells. The DNA profiles of the cell lines
were stable among the passages and during the length of time culture. This technique was demonstrated to be useful for the
detection of mixed cell lines and nucleopolyhedrovirus cell infection, using Autographa californica MNPV. The flow cytometry
gives interesting results on the cell cycle and the ploidy level; it appears as a good tool for insect cell lines characterization.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Insect cell line flow cytometry cell characterization viral infection |
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