Multistage production of Autographa californica nuclear polyhedrosis virus in insect cell cultures |
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Authors: | Martina Klöppinger Georg Fertig Elisabeth Fraune Herbert G Miltenburger |
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Institution: | (1) Institute of Zoology, Cell Biology Laboratory, Technical University, Schnittspahnstr. 3, D-6100 Darmstadt, FRG;(2) B. Braun Melsungen AG, P.O. Box 120, D-3508 Melsungen, FRG |
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Abstract: | The aim of our study was to establish an efficient system for thein vitro production of the insect pathogenic Autographa californica nuclear polyhedrosis virus in a Spodoptera frugiperda cell line. We optimized cultivation conditions for cell proliferation as well as for virus replication in a 1.5 litre stirred tank bioreactor. Cell and virus propagation were found to be optimal at a constant oxygen tension of 40%. In order to provide sufficient nutrients during virus synthesis filtration and perfusion devices were connected to the bioreactor. A virus production procedure in a repeated batch mode by using a two stage bioreactor system is described. Stage I was optimized for cell production and stage II for virus production.Abbreviations Ac-NPV
Autographa californica Nuclear Polyhedrosis Virus
- BV
Baculovirus
- MOI
Multiplicity Of Infection
- ECV
Extracellular Virus |
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Keywords: | baculovirus in vitro production perfusion cultivation |
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