Distinct volume distribution of viable and non-viable hybridoma cells: A flow cytometric study |
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Authors: | Sankar Sen Friedrich Srienc Wei-Shou Hu |
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Institution: | (1) Department of Chemical Engineering and Materials Science, University of Minnesota, 55455 Minneapolis, MN, USA;(2) Institute for Advanced Studies in Biological Process Technology, University of Minnesota, 55108 St. Paul, MN, USA |
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Abstract: | Light scattering properties of hybridoma cells were examined with flow cytometry. Viable and dead cells form two distinct populations. The distribution of the two populations changes during a batch culture. the concentration of dead cells measured by flow cytometry correlates well to that measured by hemacytometer. The distribution based on small-angle light scattering is similar to the distribution based on volume as measured by Elzone particle counter. It thus appears that viable cells form the population with a larger mean cell volume. The results also indicate that the volume of viable cells decreases during the cultivation while that of dead cells remains relatively constant. |
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Keywords: | hybridoma cell volume cell culture flow cytometry |
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