Chickpea protein hydrolysate as a substitute for serum in cell culture |
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Authors: | Julio Girón-Calle Javier Vioque Justo Pedroche Manuel Alaiz María M Yust Cristina Megías Francisco Millán |
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Institution: | (1) Instituto de la Grasa (Consejo Superior de Investigaciones Científicas), Avenida Padre García Tejero, 4, 41012 Sevilla, Spain |
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Abstract: | The growth of mammalian cells in vitro requires the use of rich culture media that are prepared by combining serum with specific
nutrient formulations. Serum, the most expensive component of culture media, provides a complex mixture of growth factors
and nutrients. Protein hydrolysates that can support in vitro cell growth and eliminate or reduce the need to use serum have
been obtained from different sources. Here we describe the use of two food grade proteases to produce a chickpea protein hydrolysate
that has been added to cell culture medium in order to determine whether it can be used as a substitute for serum. Medium
containing the hydrolysate has been tested using two human cells lines: the monocytic THP-1 cell line which grows in suspension,
and the epithelial Caco-2 cell line which grows as a monolayer. The chickpea protein hydrolysate was a good substitute for
serum in the first case, but did not allow growth of Caco-2 cells. Supplementation of culture media with this inexpensive
and safe hydrolysate would greatly reduce the cost of cell culture. |
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Keywords: | Chickpea Protein hydrolysates Cell culture THP-1 Caco-2 Serum substitute |
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