Explant organ culture: A review |
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| Authors: | James H Resau Kosaku Sakamoto John R Cottrell Eric A Hudson Stephen J Meltzer |
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| Institution: | (1) Department of Pathology, School of Medicine, University of Maryland at Baltimore, 21201 Baltimore, MD, USA;(2) Department of Medicine (G.I. Division), School of Medicine, University of Maryland at Baltimore, 21201 Baltimore, MD, USA;(3) Graduate Program in Molecular and Cell Biology, University of Maryland at Baltimore, 21201 Baltimore, MD, USA;(4) Veterans Affairs Hospital, 21218 Baltimore, MD, USA |
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| Abstract: | Organ explant culture models offer several significant advantages for studies of patho-physiologic mechanisms like cell injury, secretion, differentiation and structure development. Organs or small explants/slices can be removed in vivo and maintained in vitro for extended periods of time if careful attention is paid to the media composition, substrate selection, and atmosphere. In the case of human tissues obtained from autopsy or surgery, additional attention must be paid to the postmortem interval, temperature, hydration, and cause of death. Explant organ culture has been effectively utilized to establish outgrowth cell cultures and characterize the histiotypic relationships between the various cell types within an organ or tissue.J. Resau is a visiting scientist at the NCI-LMO-DCE in Frederick, MD 21702, U.S.A.K. Sakamoto is a visiting scientist from the Department of Surgery, Gunma University School of Medicine, Maebashi, Japan |
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| Keywords: | Organ culture human epithelium colon |
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