Cell cycle,cell size and mitochondrial activity of hybridoma cells during batch cultivation |
| |
Authors: | M Al-Rubeai S Chalder R Bird A N Emery |
| |
Institution: | (1) SERC Centre for Biochemical Engineering, School of Chemical Engineering, University of Birmingham, PO Box 363, B15 2TT Birmingham, UK;(2) Department of Immunology, University of Birmingham, PO Box 363, B15 2TT Birmingham, UK |
| |
Abstract: | Cell cycle, cell size and rhodamine 123 fluorescence in cell populations of two batch cultures were analysed and quantified with a fluorescence-activated cell sorter (FACS). Two cultures derived from either exponential or stationary phase innocula were investigated in order to demonstrate the dependency of the subsequent cell growth on innoculum condition. The results demonstrated that the level of activity of cells in the innoculum culture could have a significant effect on cellular activity during the initial phase of the inoculated culture, as it advances through its growth cycle. Positive correlation was found between the cell size and mitochondrial activity (as measured by rhodamine 123 uptake) with S and G2 fractions as the cell progressed through the cell cycle. The enumeration of the fractions of cell cycle phases has helped in prediction of the changes in cell numbers following perturbation of the culture condition. |
| |
Keywords: | hybridoma cell culture cell cycle flow cytometry cell size mitochondria |
本文献已被 SpringerLink 等数据库收录! |
|