Investigations on in vitro anti-carcinogenic potential of <Emphasis Type="SmallCaps">l</Emphasis>-carnosine in liver cancer cells |
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Authors: | Minghui Ding Guihua Jiao Haizhou Shi Yanrong Chen |
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Institution: | 1.Department of Hepatobiliary Surgery,Qingdao Municipal Hospital,Qingdao,China;2.Department of Emergency Care,First People’s Hospital of Jinan,Jinan,China;3.Department of General Surgery,Binzhou Central Hospital,Binzhou,China;4.Department of Neurology,Binzhou Central Hospital,Binzhou,China |
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Abstract: | This study was carried out to investigate the anti-carcinogenic effect of l-carnosine in human carcinoma cells (SNU-423). The SNU-423 cancer cells were cultured at a density of 2 × 104 cells/well in Dulbecco modified Eagle medium. After 24 h of adherence, the cells were treated with l-carnosine (0.2 and 1 mg/mL) for 48 h. Then, cell viability was assessed by sulforhodamine assay, while mitochondrial dysfunction was measured by fluorescence microscopy using chromatin-specific dye Hoechst 33258. Intracellular levels of ROS were assayed by fluorescence spectroscopy with 2′,7′-dichlorofluorescein diacetate (DCFDA). l-Carnosine significantly inhibited the growth of the SNU-423 cells (p < 0.05). The inhibitory effect of l-carnosine was confirmed by results from mitochondrial fragmentation assay. The relative fluorescent unit was increased in a dose-dependent manner by l-carnosine, with values of 79.43, 186.87 and 400.89 for 0.6, 0.8 and 1 mg/mL of l-carnosine, respectively (p < 0.05). These results demonstrate that l-carnosine exerts anti-carcinogenic effects in human liver cancer cells. |
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