Friedelane,isolated from <Emphasis Type="Italic">Pouzolzia indica</Emphasis> Gaud. exhibits toxic effect against melanoma |
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| Authors: | Debasish Maiti Ashish Kumar Singha Chaitali Sarkar Soham Sarkar Indrajit Sil Sarma Kuntal Manna Biswanath Dinda |
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| Institution: | 1.Immunology Microbiology Lab, Department of Human Physiology,Tripura University,Agartala,India;2.Wilson’s School,Wallington,UK;3.Tripura State Forensic Science Laboratory,Narasingarh, Agartala,India;4.Department of Pharmacy,Tripura University,Agartala,India;5.Department of Chemistry,Tripura University,Agartala,India |
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| Abstract: | Melanoma is a predominant cause of skin cancer-related deaths. It was reported that, the methanolic extract of Pouzolzia Indica (P. indica) on chromatography gave five compounds (1-hentriacontanyl palmitate, myricyl alcohol, 6,7-dimethoxycoumarin, trichadonic acid and friedelane), which inhibited the acute promyelocytic leukemia cell lines, NB4, and HT93A. Friedelane was extracted as active compound from methanolic extract of P. indica. In this study, friedelane was tested on murine metastatic B16F10 and B16BL6 melanoma cell lines. To achieve the target, the cell viability using trypan blue exclusion, acridine orange/EtBr staining and cell cytotoxicity were tested using MTT assay. Caspase-3, caspase-9, Cyt-c, BAD and Bax protein were assayed to evidence the apoptosis induction. The compound friedelane shows potent cytotoxic effect against metastatic melanoma mouse cell lines in 10 µg/ml concentration. |
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