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A whole-mechanical method to establish human embryonic stem cell line HN4 from discarded embryos
Authors:Bin Li  Lan Xu  Wei-Ying Lu  Wen Xu  Mei-Hong Wang  Ke Yang  Juan Dong  Xiao-Yan Ding  Yuan-Hua Huang
Institution:(1) Reproductive Medical Center of Hainan Province, Hainan Medical College, 570102 Haikou, China;(2) Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031 Shanghai, China;(3) Present address: Obstetrics & Gynecology Hospital of Fudan University, 200011 Shanghai, China
Abstract:
Keywords:Human stem cells  ICM mechanical isolation  Mixed feeder layer  Neural differentiation
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