Combined effect of temperature and light intensity on growth and extracellular polymeric substance production by the cyanobacterium Arthrospira platensis |
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Authors: | Lamia Trabelsi Hatem Ben Ouada Hassen Bacha Mohamed Ghoul |
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Institution: | (1) Laboratoire de Biodiversité et de Biotechnologie Marine, Institut National des Sciences et Technologies de la Mer, BP 59, Route de Khniss, Monastir, 5000, Tunisia;(2) Laboratoire de Recherches sur les Substances Biologiquement Compatibles Faculté de Medecine Dentaire, Monastir, 5000, Tunisia;(3) Laboratoire de Bioprocédés Agroalimentaires, 2, Avenue de la Foret de Haye, 54500 Vendoeuvre-les-Nancy, France |
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Abstract: | The effects of light intensity and temperature on Arthrospira platensis growth and production of extracellular polymeric substances (EPS) in batch culture were evaluated using a three-level, full-factorial
design and response surface methodology. Three levels were tested for each parameter (temperature: 30, 35, 40°C; light intensity:
50, 115, 180 μmol photons m−2 s−1). Both growth and EPS production are influenced mainly by the temperature factor but the interaction term temperature*light
intensity also had a significant effect. In addition, conditions optimising EPS production are different from those optimising
growth. The highest growth rate (0.414 ± 0.003 day−1) was found at the lowest temperature (30°C) and highest light intensity (180 μmol photons m−2 s−1) tested, no optima were detectable within the given test range. Obviously, optima for growth must be at a temperature lower
than 30°C and a light intensity higher than 180 μmol photons m−2 s−1. For EPS production, light intensity had a positive linear effect (optimum obviously higher than 180 μmol photons m−2 s−1), but for the temperature parameter a maximum effect was detectable at 35°C. |
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Keywords: | Arthrospira platensis Extracellular polymeric substance production Growth rate Light intensity Response surface methodology Spirulina Temperature |
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