Genetic engineering in marine cyanobacteria |
| |
Authors: | Tadashi Matsunaga Haruko Takeyama |
| |
Institution: | (1) Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184, Japan |
| |
Abstract: | Many species of microalgae producing useful materials have been isolated from marine environments. For their industrial application,
widely applicable and stable gene expression is required. It is necessary to establish gene transfer methods as an essential
first step in genetic manipulation. Although gene transfer techniques for cyanobacteria have been developed, only naturally
transformable strains have been used. Here, we describe recent progress made in developing gene transfer methods for marine
cyanobacteria. The following are covered: (1) transformation, (2) electroporation, (3) conjugation, (4) particle gun. A plasmid
from the marine cyanobacterium, Synechococcus sp., whose copy number is dependent on salinity, was characterized. This plasmid is being used to develop a stable and controllable
gene expression system. |
| |
Keywords: | marine cyanobacteria Synechococcus gene transfer transformation conjugation electroporation particle gun |
本文献已被 SpringerLink 等数据库收录! |
|