Isolation and linkage mapping of NBS-LRR resistance gene analogs in red raspberry (<Emphasis Type="Italic">Rubus idaeus</Emphasis> L.) and classification among 270 Rosaceae NBS-LRR genes |
| |
Authors: | Suren K Samuelian Angela M Baldo Jeremy A Pattison Courtney A Weber |
| |
Institution: | (1) New York State Agricultural Experiment Station, Cornell University, 630 West North St., Geneva, NY 14456, USA;(2) Center for Viticulture and Small Fruit Research, Florida A&M University, 6505 Mahan Drive, Tallahassee, FL 32317, USA;(3) USDA-ARS Plant Genetic Resources Unit, 630 W. North St., Geneva, NY 14456, USA;(4) Present address: Virginia Polytechnic Institute and State University, Southern Piedmont AREC, 2375 Darvills Road, Blackstone, VA 23824, USA |
| |
Abstract: | Plant R genes confer resistance to pathogens in a gene-for-gene mode. Seventy-five putative resistance gene analogs (RGAs) containing
conserved domains were cloned from Rubus idaeus L. cv. ‘Latham’ using degenerate primers based on RGAs identified in Rosaceae species. The sequences were compared to 195
RGA sequences identified from five Rosaceae family genera. Multiple sequence alignments showed high similarity at multiple
nucleotide-binding site (NBS) motifs with homology to Drosophila Toll and mammalian interleukin-1 receptor (TIR) and non-TIR
RNBSA-A motifs. The TIR sequences clustered separately from the non-TIR sequences with a bootstrap value of 76%. There were
11 clusters each of TIR and non-TIR type sequences of multiple genera with bootstrap values of more than 50%, including nine
with values of more than 75% and seven of more than 90%. Polymorphic sequence characterized amplified region and cleaved amplified
polymorphic sequence markers were developed for nine Rubus RGA sequences with eight placed on a red raspberry genetic linkage map. Phylogenetic analysis indicated four of the mapped
sequences share sequence similarity to groupTIR I, while three others were spread in non-TIR groups. Of the 75 Rubus RGA sequences analyzed, members were placed in five TIR groups and six non-TIR groups. These group classifications closely
matched those in 12 of 13 studies from which these sequences were derived. The analysis of related DNA sequences within plant
families elucidates the evolutionary relationship and process involved in pest resistance development in plants. This information
will aid in the understanding of R genes and their proliferation within plant genomes.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
| |
Keywords: | Disease resistance Resistance gene analog |
本文献已被 SpringerLink 等数据库收录! |
|