Comparing the use of leaf and cambium tissue in a single genetic study of tropical trees |
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Authors: | Sharon E Zytynska Michael F Fay and Richard F Preziosi |
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Institution: | (1) Michael Smith Building, Faculty of Life Sciences, University of Manchester, Manchester, M13 9PT, UK;(2) Jodrell Laboratory, Royal Botanic Gardens, Kew, TW9 3DS, UK;(3) Present address: Department of Ecology and Ecosystem Management, Technische Universit?t M?nchen, Freising, Germany |
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Abstract: | Amplified fragment length polymorphisms (AFLPs) are a useful molecular tool for studying species with little available genetic
information; however, since universal primers are used contaminant DNA from non-target organisms may also be amplified. Cambium
tissue may contain fewer biotic contaminants or plant defense chemicals, than the more commonly used leaf tissue, and therefore
be more suitable for use as a source of DNA when using universal primers. On the other hand, cambium tissue can be difficult
to identify, yields low DNA and requires the bark of the tree to be damaged, thereby increasing the risk of introducing disease.
We show that within two tropical tree species, there are few differences between AFLP profiles obtained from either cambium
or leaf tissue from the same tree. We studied 50 Brosimum alicastrum individuals at 119 AFLP loci and 40 Swietenia macrophylla individuals at 112 AFLP loci. The matrix of S?rensen similarity indices between individual AFLP profiles for cambium samples
was strongly correlated to the matrix for leaf samples in each species (Mantel test; B. alicastrum r = 0.815, P < 0.001; S. macrophylla r = 0.895, P < 0.001). The phylogenetic relationship between the trees studied did not differ dependent on tissue type used and therefore
shows that both tissues can be used within a single study without introducing substantial error. |
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