Development of retrotransposon primers and their utilization for germplasm identification in Diospyros spp. (Ebenaceae) |
| |
Authors: | Xiaoyun Du Qinglin Zhang Zhengrong Luo |
| |
Institution: | (1) Department of Pomology, Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forest Science, Huazhong Agricultural University, Wuhan, 430070, China |
| |
Abstract: | Retrotransposons play an important role in plant genetic instability and genome evolution. Retrotransposon-based molecular
markers are valuable tools to reveal the behavior of retrotransposons in their host genome. In this study, suppression polymerase
chain reaction was used, for the first time, to develop retrotransposon long terminal repeat (LTR) and polypurine tract (PPT)
primers in Japanese persimmon (Diospyros kaki Thunb.), which were then employed for germplasm identification by means of interretrotransposon-amplified polymorphism (IRAP),
sequence-specific amplified polymorphism (SSAP) and retrotransposon-microsatellite-amplified polymorphism (REMAP) molecular
markers. The results showed that 16 out of 26 primers produced expected amplifications and abundant polymorphisms by IRAP
in 28 genotypes of Diospyros. Moreover, some of these primers were further successfully used in REMAP and SSAP analysis. Each type of molecular markers
produced unique fingerprint in 28 genotypes analyzed. Among the primers/primer combinations, two IRAP primers and four SSAP
primer combinations could discriminate all of the germplasm solely. Further comparative analysis indicated that IRAP was the
most sensitive marker system for detecting variability. High level of retrotransposon insertion polymorphisms between bud
sports were detected by IRAP and SSAP, and the primers/primer combinations with powerful discrimination capacity for two pairs
of bud sports lines were further obtained. Additionally, possible genetic relationships between several Japanese persimmon
were discussed. To our knowledge, this is the first report on the development of retrotransposon LTR and PPT primers in Diospyros, and the retrotransposon primers developed herein might open new avenue for research in the future. |
| |
Keywords: | Diospyros kaki Thunb Suppression PCR RNaseH-LTR Retrotransposon SSAP IRAP REMAP |
本文献已被 SpringerLink 等数据库收录! |
|