| 短尾蝮蛇IRF-2基因的克隆和特征分析 |
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| 引用本文： | 甘小妮,陈新文.短尾蝮蛇IRF-2基因的克隆和特征分析[J].四川动物,2011,30(4). |
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| 作者姓名： | 甘小妮 陈新文 |
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| 作者单位： | 1. 中国科学院武汉病毒研究所,武汉,430072;中国科学院水生生物研究所,武汉,430072
2. 中国科学院武汉病毒研究所,武汉,430072 |
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| 摘 要： | 报道了短尾蝮蛇Gloydius brevicaudus干扰素调节因子2基因(IRF-2)的克隆和cDNA全序列测定分析.目前除在爬行类外,IRF-2基因在大部分的脊椎动物如鱼类、两栖类、鸟类和哺乳类都有报道.为了获得爬行类IRF-2基因的全序列,从短尾蝮蛇的肾、脾、肝和肠4种组织中使用Trizol试剂盒提取了总RNA.从已知的IRF-2基因序列比对设计了简并引物来扩增保守片段.最后使用RACE方法得到IRF-2的cDNA全序列.结果 显示短尾蝮蛇的IRF-2基因开放阅读框包含有978个核苷酸,编码326个氨基酸,其3′UTR包含137个核苷酸.与脊椎动物其他四足动物序列比对分析还发现,短尾蝮蛇的IRF-2基因和推导的氨基酸序列都非常保守,与大部分四足动物的IRF-2相似.从其氨基酸序列中可以辨别出DBD、IDA2和transactivating domain等大部分元件和阻遏模体.
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| 关 键 词： | 蝮蛇 爬行类 克隆和特征分析 |
Cloning and Character Analyses of IRF-2 Gene for Gloydius brevicaudus |
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| GAN Xiao-ni,CHEN Xin-wen.Cloning and Character Analyses of IRF-2 Gene for Gloydius brevicaudus[J].Sichuan Journal of Zoology,2011,30(4). |
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| Authors: | GAN Xiao-ni CHEN Xin-wen |
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| Institution: | GAN Xiao-ni1,2,CHEN Xin-wen1(1.Institute of Wuhan Virology,Chinese Academy of Sciences,Wuhan 430072,China,2.Institute of Hydrobiology,China) |
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| Abstract: | IRF-2 gene have been reported in most of vertebrates such as fishes,amphibians,birds and mammals but not in reptiles.For the purpose of obtaining the cDNA sequence of reptile IRF-2,total RNA was isolated from the tissues of kidney,spleen,liver,and intestine of a viper(Gloydius brevicaudus) using Trizol kit.The degenerate primers to obtain IRF-2 were designed by comparing known IRF-2 sequences.RACE method also was applied to obtain the complete cDNA sequence of IRF-2 gene.The complete cDNA sequence of G.brev... |
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| Keywords: | IRF-2 |
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