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| 大熊猫精浆蛋白的SDS-PAGE电泳研究 | |
| 引用本文： | 张明,鲜红,侯蓉,朱庆,郑鸿培.大熊猫精浆蛋白的SDS-PAGE电泳研究[J].四川动物,2006,25(4):735-738. |
| 作者姓名： | 张明 鲜红 侯蓉 朱庆 郑鸿培 |
| 作者单位： | 1. 四川农业大学动物科技学院繁殖生物技术实验室,四川雅安,625014;成都大熊猫繁育基地濒危野生动物,繁殖与保护遗传四川省重点实验室 2. 成都市妇产科医院生殖不孕研究所 3. 成都大熊猫繁育基地濒危野生动物,繁殖与保护遗传四川省重点实验室 4. 四川农业大学动物科技学院繁殖生物技术实验室,四川雅安,625014 |
| 摘 要： | 本试验采用SDS-PAGE对大熊猫精浆进行电泳并对电泳条带进行分析，探讨特异条带与大熊猫精液质量的关系。结果显示：大熊猫精浆SDS-PAGE电泳共分离得到12条蛋白谱带，其中6个条带为所有个体大熊猫精浆样品所共有，共有条带的相对量在不同个体之间不存在差异（P〉0．05）。试验中部分大熊猫精浆出现的特异蛋白带和共有带蛋白含量与精液质量无相关性。 |
| 关 键 词： | 大熊猫 精浆 电泳 精液质量 聚类分析 |
| 文章编号： | 1000-7083（2006）04-0735-04 |
| 收稿时间： | 2006-01-22 |
| 修稿时间： | 2006年1月22日 |
Study on Giant Panda (Ailuropoda melanoleuca) Seminal Plasma Protein Electrophoresed with SDS-PAGE |
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| ZHANG Ming,XIAN Hong,HOU Rong,ZHU Qing,ZHENG Hong-pei.Study on Giant Panda (Ailuropoda melanoleuca) Seminal Plasma Protein Electrophoresed with SDS-PAGE[J].Sichuan Journal of Zoology,2006,25(4):735-738. | |
| Authors: | ZHANG Ming XIAN Hong HOU Rong ZHU Qing ZHENG Hong-pei |
| Abstract: | In the study,giant panda seminal plasma protein was separated by SDS-PAGE and their relationship with semen characteristics was analyzed.The results are as follows: Twelve protein bands in giant panda seminal plasma were identified by SDS-PAGE,and the molecular weights were 74.9,59.6,56.2,54.7,47.3,38.2,30.8,23.8,18.4,16.8,14.0 and 13.4 kDa respectivelyr.Six of them(74.9,59.6,56.2,47.3,30.8 and 13.4 kDa) were present in all samples and relative quantity was no different among them.However,no relationship was found among the abnormal protein bands appeared in several samples and semen characteristics in our experiment. |
| Keywords: | giant panda seminal plasma SDS-PAGE semen quality clustering analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |