| 长薄鳅外周血细胞的显微结构和细胞化学特征研究 |
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| 引用本文： | 黄小铭,张耀光,江星,李凤杰,金丽.长薄鳅外周血细胞的显微结构和细胞化学特征研究[J].四川动物,2012,31(1):59-63. |
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| 作者姓名： | 黄小铭 张耀光 江星 李凤杰 金丽 |
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| 作者单位： | 西南大学生命科学学院,淡水鱼类资源与生殖发育教育部重点实验室,水产科学重庆市市级重点实验室,重庆400715 |
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| 基金项目： | 中央高校基本科研业务费专项资金资助项目，西南大学博士基金，公益性行业(农业)科研专项经费 |
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| 摘 要： | 长薄鳅外周血细胞可分为红细胞、中性粒细胞、单核细胞、淋巴细胞和血栓细胞.在数量上,中性粒细胞、单核细胞、淋巴细胞和血栓细胞占白细胞总数的百分比分别是17.06%、5.83%、28.16%和48.94%.细胞化学染色显示所有白细胞均含有糖原物质,所有红细胞均不含酸性磷酸酶,中性粒细胞、单核细胞、淋巴细胞和血栓细胞均含有酸性磷酸酶.非特异件酯酶染色显示单核细胞呈阳性反应,中性粒细胞、淋巴细胞和血栓细胞均为部分呈阳性反应.所有细胞的碱性磷酸酶、过氧化物酶、苏丹黑显色反应均呈阴性.
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| 关 键 词： | 长薄鳅 血细胞 显微结构 细胞化学 |
Structures and Cytochemical Characteristics of Peripheral Blood Cells in Leptobotia elongata |
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| HUANG Xiao-ming,ZHANG Yao-guang,JIANG Xing,LI Feng-jie,JIN Li.Structures and Cytochemical Characteristics of Peripheral Blood Cells in Leptobotia elongata[J].Sichuan Journal of Zoology,2012,31(1):59-63. |
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| Authors: | HUANG Xiao-ming ZHANG Yao-guang JIANG Xing LI Feng-jie JIN Li |
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| Institution: | *(Key Laboratory of Freshwater Fish Reproduction and Development(Southwest University),Ministry of Education;The Key Laboratory of Aquatic Science of Chongqing,School of Life Science,Southwest University,Chongqing 400715,China) |
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| Abstract: | The peripheral blood cells of Leptobotia elongata consist of erythrocyte,neutrophilic granulocyte,monocyte,lymphocyte and thrombocyte.The percentage of neutrophilic granulocyte,monocyte,lymphocyte and thrombocyte in leucocytes is 17.06%,5.83%,28.16% and 48.94%,respectively.The results of cytochemical staining showed that there were glycogen in all leucocytes.All erythrocytes are without acid phoshpatase,but acid phoshpatase were existed in neutrophilic granulocytes,monocytes,lymphocytes and thrombocytes.α-naphthyl acetate esterase staining indicated that monocytes revealed positive reactions which was only partial in neutrophilic granulocytes,lymphocytes and thrombocytes.The color reactions of alkaline phoshpatase,peroxidase and Sudan Black B in all blood cells were negative. |
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| Keywords: | Leptobotia elongata blood cells microstructure cytochemistry |
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