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| 藏酋猴微卫星富集文库的构建及微卫星分子标记的筛选 | |
| 引用本文： | 贾小东,张修月,王红星,王中凯,尹湧华,岳碧松.藏酋猴微卫星富集文库的构建及微卫星分子标记的筛选[J].四川动物,2012,31(1):39-44. |
| 作者姓名： | 贾小东 张修月 王红星 王中凯 尹湧华 岳碧松 |
| 作者单位： | 1. 四川省濒危野生动物保护生物学重点实验室,四川大学生命科学学院,成都610064;绵阳师范学院生命科学与技术学院,四川绵阳621000 2. 四川省濒危野生动物保护生物学重点实验室,四川大学生命科学学院,成都610064 3. 四川省医学科学院·四川省人民医院实验动物研究所,成都610212 |
| 基金项目： | 成都大熊猫繁殖研究基金 |
| 摘 要： | 通过磁珠富集法构建了藏酋猴AC重复和AAAG重复的微卫星富集文库,分离微卫星序列并对其进行分析。将藏酋猴基因组DNA经Sau3AI酶切后纯化回收,连接特定接头。用生物素标记的探针与酶切片段杂交,捕获300～1000bp片段,随后将获得的片段连接到pMD-19T载体上,转化至JM109中,成功构建藏酋猴微卫星富集文库。(AC)n富集文库和(AAAG)n富集文库的阳性克隆率分别为50%和10%左右。根据测序得到的48个微卫星序列成功设计了24对引物,最终筛选出6个微卫星标记,这些标记将为藏酋猴的遗传多样性研究、圈养种群结构的分析和遗传图谱的构建等奠定一定的基础。 |
| 关 键 词： | 藏酋猴 富集文库 微卫星 |
Construction of Microsatellite-enriched Library and Isolation of Microsatellite Markers in Macaca thibetana |
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| JIA Xiao-dong,ZHANG Xiu-yue,WANG Hong-xing,WANG Zhong-kai,YIN Yong-hua,YUE Bi-song.Construction of Microsatellite-enriched Library and Isolation of Microsatellite Markers in Macaca thibetana[J].Sichuan Journal of Zoology,2012,31(1):39-44. | |
| Authors: | JIA Xiao-dong ZHANG Xiu-yue WANG Hong-xing WANG Zhong-kai YIN Yong-hua YUE Bi-song |
| Institution: | 1*(1.Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife,College of Life Sciences,Sichuan University,Chengdu 610064,China;2.College of Resource and Environmental Engineering,Mianyang Normal University,Mianyang,Sichuan Province 621000,China;3.Institute of Lab Animal of Sichuan Medicine Academy,Sichuan Provincial People’s Hospital,Chengdu 610212,China) |
| Abstract: | Microsatellite-enriched libraries of AC-repeats and AAAG-repeats in Macaca thibetana were constructed using an enrichment method with biotin-labeled probes and streptavidin-coated magnetic beads.The ratios of positive clones containing(AC)n and(AAAG)n enriched libraries were approximately 50% and 10%,respectively.Total 48 microsatellite sequences were obtained by sequencing positive clones.According to sequencing results,24 pairs of primers were designed,and of them 6 markers were optimized.The 6 microsatellite makers of M.thibetana obtained from this study would be significant in future study,such as population structure analyses,paternity testing,and genetic mapping. |
| Keywords: | Macaca thibetana enriched library microsatellite |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |