10种植物乙醇提取物对金银花蚜虫的生物活性研究

采用微量滴定法测定了10种植物乙醇提取物对金银花蚜虫的生物活性.结果表明,10种植物乙醇提取物对金银花蚜虫均有一定的驱避活性和毒杀作用.在10 mg/mL的浓度下,臭椿、曼陀罗和桂花的提取物对金银花蚜虫的驱避作用较强,其在12 h的驱避率分别为78.74％、77.54％、75.11％;夏枯草提取物的驱避效果最弱,12 h的驱避率为58.53％.随着处理时间的延长,各植物提取物的驱避效果均呈下降趋势；印楝提取物的毒杀活性最强,72 h后的校正死亡率达68.30％；曼陀罗和苦瓜的作用次之,72 h后的校正死亡率分别为66.41％和63.70％；夏枯草提取物的作用最低,72 h后的校正死亡率仅为26.47％.进一步的毒力分析表明,印楝提取物对金银花蚜虫的毒力较强,24h的Lc.值为11.0836 mg,/mL.

Research on Alcohol Extracts from 10 Plants to Bioactivity of Honeysuckle Aphid

Bioactivity of alcohol extracts from 10 plants on the honeysuckle aphid was tested by trace titration method in the laboratory. The results showed that alcohol extracts from 10 plants had a certain repellent activity and insecticidal activity on honeysuckle aphid. At the dosage of 10 mg/mL, repellent activity of extract from heaven tree, stramonium and sweet olive were stronger than others, their repellent rate were 78.74%, 77.54% and 75.11% respectively after 12 h, repellent activity of extract from prunella was the lowest, which was 58.53%. The repellent activity will reduce with the extending of treated time. Insecticidal activity of extract from azadirachta indica was the strongest, the corrected mortality reached 68.30% after 72 h. The insecticidal activity of extracts from stramonium and balsam pear were the second, the corrected mortality of them were 66.41% and 63.07% respectively, the corrected mortality of extract from prunella was the lowest, which was 26.47%. By further toxicity analysis, we found that toxicity of extract from azadirachta indica was the strongest, whose LC50 was 11.0836 mg/mL after 24 h.