长鬣蜥胃肠道5-羟色胺细胞的免疫组织化学定位

研究长鬣蜥消化道内含有的5一羟色胺（5-HT）内分泌细胞的位置和形态，采用ABC（avidin—biotin—peroxidase complex）免疫组织化学法，应用5-HT特异性抗血清，对其消化道内的5-HT细胞进行免疫化学定位。结果：5-HT细胞从胃部到直肠的胃肠道各段均有分布，细胞密度分布以胃部最高，十二指肠次之，回肠最低。5-HT细胞的形态呈圆形、椭圆形和锥体形等，其中胃体部和直肠部以圆形和椭圆形为主，小肠部以锥体形为主；5-HT细胞广泛分布于上皮基部、上皮细胞之间、腺泡上皮及腺泡之间，有时可见于固有膜内。结论：5-HT细胞的密度分布与其食性、食物组成和生活环境有关，5-HT细胞的形态与其内、外分泌是相适应的。

Immunohistochemical Localization of 5-HT Cells in Gastrointestinal Tract of Physignathus cocincinus

To study the distributed location and morphology of 5-HT endocrine cells in the digestive tract of Physignathus cocincinus,anti-5-HT serum and the method of the ABC(avidin-biotin-peroxidase complex)immunhistochemical technique were used.Results:5-HT cells distribute throughout the gastrointestinal tract,from stomach to rectum,and the distributive density of 5-HT cells is the maximum in stomach,the second in duodenum and the minimum in ileum.The shapes of 5-HT cells are diverse and there are round,ellipse,shuttle and so on.Majority of the 5-HT cells are round and ellipse in stomach and rectum,but most of them are shuttle in small intestine.The 5-HT cells are found at the basal region of epithelial cells,between gastrointestinal epithelial cells,between glandular epithelial cells or in the lamina propria.Conclusion The distributive density of 5-HT cells of Physignathus cocincinus is related to its feeding habit,food component and living environment.And morphologies of 5-HT cells and endocrine and exocrine functions are fit mutually.