基于核基因RAG1部分序列探讨菊头蝠科和蹄蝠科的系统发育关系

应用核基因重组激活基因1(Recombination activating gene,RAG1)部分序列对贵州9种菊头蝠和5种蹄蝠的系统发育关系进行了研究,运用贝叶斯法(Bayesian inference,BI)、最大似然法(Maximum likelihood,ML)和邻接法(Neighbor-joining,NJ)构建了系统进化树。研究结果表明:1.菊头蝠科和蹄蝠科形成两个平行的分支,支持它们是两个平行科的结论。2.马铁菊头蝠是菊头蝠科中第一个独立出来的亚分支,与其余8种菊头蝠的亲缘关系最远;Rhinolophus sp.与大菊头蝠聚合为一支,推测Rhinolophus sp.可能是大菊头蝠,或者是大菊头蝠的近缘种,它们是继马铁菊头蝠之后分离出来的第二亚分支;中华菊头蝠、栗黄菊头蝠、中菊头蝠聚合为第三亚分支,贵州菊头蝠、大耳菊头蝠、菲菊头蝠聚合为第四亚分支,此两分支形成姊妹群。3.在蹄蝠科的亚分支中,三叶蹄蝠、小蹄蝠、普氏蹄蝠依次分离出来,最后分出的是大蹄蝠和中蹄蝠。

Phylogenetics of Rhinolophidae and Hipposideridae Based on Partial Sequences of the Nuclear RAG1 Gene

We examined the phylogentic relationship among nine species of Rhinolophids and five species of Hipposideros in Guizhou province,China by partially sequencing of nuclear RAG1 gene and produced evolutionary trees using Bayesian inference(BI),Maximum likelihood(ML) and Neighbor-joining(NJ) methods.The results suggested:1.Rhinolophidae and Hipposideridae were divided into two clades,supporting that they were two parallel families.2.R.ferrumequinum was separated from the Rhinolophidae firstly and the farthest relationship to other Rhinolophids;R.sp and R.luctus consisted together to infer that R.sp may be R.luctus or is closely related,as the second sub-clade separated from the other Rhinolophids;R.sinicus,R.rouxii and R.affinis consisted the third sub-clade;R.rex,R.macrotis and R.pusillus consisted the forth sub-clade,which were sister group.3.In the sub-clades of Hipposideridae,A.stoliczkanus,H.pomona and H.pratti separated as single sub-clade.H.armiger and H.larvatus separated from the other Hipposideros at last.