| 马卵母细胞体外成熟的初步研究 |
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| 引用本文： | 崔亮,赵高平,吴凯峰,巴特尔,周欢敏.马卵母细胞体外成熟的初步研究[J].四川动物,2011,30(1). |
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| 作者姓名： | 崔亮 赵高平 吴凯峰 巴特尔 周欢敏 |
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| 作者单位： | 1. 内蒙古农业大学动物科学学院,呼和浩特,010018
2. 内蒙古农业大学生命科学学院,呼和浩特,010018 |
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| 基金项目： | 内蒙古农业大学校内《体细胞克隆马》项目资助 |
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| 摘 要： | 采用抽吸法和切割法两种采卵方法收集马卵母细胞,显示采用切割法的卵母细胞回收率为83%,高于抽吸法的回收率46.5%(P＜0.05),在卵母细胞的成熟上,使用M199和DMEM/F12两种培养液为基础液的成熟体系,紧凑型(Cp)COCs和扩展型(Ex)COCs在以M199为基础液和以DMEM/F12为基础液的培养液中的卵母细胞成熟率分别为41.7%和64.7%、46.7%和66.7%,差异不显著(P＞0.05).两种培养体系中成熟的Cp COCs采用Ionomycin与6-DMAP和CHX联合激活,在以M199和以DMEM/F12为基础液的培养液中成熟的卵母细胞的卵裂率分别为45%和57.1%,差异不明显(P＞0.05).
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| 关 键 词： | 马卵母细胞 体外成熟 孤雌激活 |
Study on in vitro Maturation of Equine Oocytes |
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| CUI Liang,ZHAO Gao-ping,WU Kai-feng,BA Te-er,ZHOU Huan-min.Study on in vitro Maturation of Equine Oocytes[J].Sichuan Journal of Zoology,2011,30(1). |
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| Authors: | CUI Liang ZHAO Gao-ping WU Kai-feng BA Te-er ZHOU Huan-min |
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| Institution: | CUI Liang1,ZHAO Gao-ping1,WU Kai-feng2,BA Te-er1,ZHOU Huan-min2(1.College of Animal Science and Veterinary Medicine,Inner Mongolia Agriculture University,Hohhot 010018,China,2.College of Life Science,China) |
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| Abstract: | In this study,two different methods(aspiration and incision)were evaluated for efficiency of equine oocyte collection.Two maturation media were used for in vitro maturation of equine oocytes.In medium 1,the basic medium was M199;in medium 2,the basic medium was DMEM/F12.The results showed that the incision method was better than the aspiration method for efficiency of equine oocyte collection(83% vs 46.5%)(P<0.05).There were no significant differences in the maturation rate observed among the two different ... |
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| Keywords: | equine oocytes in vitro maturation parthenogenetic activation |
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