| 猴泡沫病毒SFV-1前病毒pol基因克隆及序列分析 |
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| 引用本文： | 匡德宣,罕圆圆,李春花,李跟党,陈瑜,唐东红.猴泡沫病毒SFV-1前病毒pol基因克隆及序列分析[J].四川动物,2009,28(4). |
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| 作者姓名： | 匡德宣 罕圆圆 李春花 李跟党 陈瑜 唐东红 |
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| 作者单位： | 1. 中国医学科学院/北京协和医学院医学生物学研究所,昆明,650118
2. 云南省中医学院 |
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| 摘 要： | 采用nested-PCR从猕猴肾组织细胞中获得同源性较高、具有高度保守序列的、长度为465 bp 大小的猴泡沫病毒SFV-1的 pol 基因的cDNA,将其克隆到pMD18-T载体中,并对其进行序列分析,分析结果表明所克隆的基因片断为猴泡沫病毒SFV-1 pol 基因片段,与文献中已发表的来源于灵长类动物SFV-1、SFV-1A、SFV-1B、SFV-2、 SFVmac、SFVkm的 pol 基因核苷酸序列进行比对,显示SFVkm1与上述病毒 pol 基因核苷酸同源性分别为91.06%、90.59%、90.82%、90.21%、89.41%、91.53%.对 pol 基因克隆和序列分析是了解和掌握SFV病毒分子流行病学及其变异趋势的重要手段,7 种不同基因型的泡沫病毒的系统进化树分析显示了SFVkm1与它们之间的相互联系.
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| 关 键 词： | 猴泡沫病毒 pol基因 分子克隆 序列分析 |
Clone and Sequence Analysis of the pol Gene of Simian Foamy Virus Type 1 Provirus |
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| KUANG De-xuan,HAN Yuan-yuan,LI Chun-hua,LI Geng-dang,CHENG Yu,TANG Dong-hong.Clone and Sequence Analysis of the pol Gene of Simian Foamy Virus Type 1 Provirus[J].Sichuan Journal of Zoology,2009,28(4). |
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| Authors: | KUANG De-xuan HAN Yuan-yuan LI Chun-hua LI Geng-dang CHENG Yu TANG Dong-hong |
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| Institution: | 1.Institute of Medical Biology;Chinese Academy of Medical Science /Peking Union Medical College;Kunming 650118;China;2.Yunnan University of Traditional Chinese Medicine |
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| Abstract: | A 465 bp DNA segment was obtained by nested PCR from kidney culture of rhesus monkey using primers homologies to highly conserved portions of pol gene of simian foamy virus type 1 provirus,and clone it into the vector pMD18-T for sequencing.The pol gene was identified by comparison with other primate foamy viruses.The sequence comparison with the pol gene of SFV-1,SFV-1A,SFV-1B,SFV-2,SFVmac,SFVkm showed nucleotide homologies of 91.06%,90.59%,90.82%,90.21%,89.41%,91.53% respectively.We conclude that limited ... |
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| Keywords: | simian foamy virus pol gene DNA cloning sequence analysis |
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