| 馆藏陈旧蟹类标本(包含模式种)基因组DNA提取和PCR扩增方法改进及所获序列的初步分析 |
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| 引用本文： | 张代臻,唐伯平,杨思谅.馆藏陈旧蟹类标本(包含模式种)基因组DNA提取和PCR扩增方法改进及所获序列的初步分析[J].四川动物,2008,27(1):31-34. |
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| 作者姓名： | 张代臻 唐伯平 杨思谅 |
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| 作者单位： | 1. 江苏省滩涂生物资源与环境保护重点建设实验室,盐城师范学院,江苏盐城,224002
2. 北京自然博物馆 |
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| 基金项目： | 国家自然科学基金
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盐城师范学院高层次人才引进启动基金 |
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| 摘 要： | 若能解决馆藏陈旧标本DNA提取及PCR扩增等问题,利用模式标本或地模标本解决分类学问题将更具有说服力.本研究通过改进实验方法、优化反应条件成功地从馆藏陈旧蟹类标本中获得了靶向目的序列,并对所获序列的变异特点作了初步分析,旨在为通过改进后的方法成功获得馆藏模式标本、地模标本的目的DNA序列,以解决目前蟹类分类系统中存在的问题提供一定实验基础.
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| 关 键 词： | 模式标本 地模标本 巢式PCR 蟹 |
| 文章编号： | 1000-7083（2008）01-0031-04 |
| 收稿时间： | 2007-05-27 |
| 修稿时间： | 2007-09-04 |
Improved Methods of Genome DNA Extraction and PCR for Old Crab Samples Including Lectotype of Museum |
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| ZHANG Dai-zhen,TANG Bo-ping,YANG Si-liang.Improved Methods of Genome DNA Extraction and PCR for Old Crab Samples Including Lectotype of Museum[J].Sichuan Journal of Zoology,2008,27(1):31-34. |
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| Authors: | ZHANG Dai-zhen TANG Bo-ping YANG Si-liang |
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| Abstract: | It will be more convictive using lectotype or topotype to clarfy taxon problem if the methods of DNA extraction and PCR from old samples are optimized. In this study, nuclear target sequences were successfully obtained from old crabs preserved in Beijing Natural History Museum using improved methods. And variable characters of obtained sequences were analyzed. The improved methods will provide some experimental base in clarifying crabs taxon problems using lectotype and topotype preserved in museum. |
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| Keywords: | lectotype topotype nested-PCR crab |
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