| 獐不同组织材料DNA提取的有效方法 |
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| 引用本文： | 陈珉,张恩迪.獐不同组织材料DNA提取的有效方法[J].四川动物,2006,25(3):481-484. |
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| 作者姓名： | 陈珉 张恩迪 |
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| 作者单位： | 华东师范大学生命科学学院,上海,200062 |
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| 摘 要： | 根据野外采集的獐肌肉、皮张、毛发、血迹、骨骼和粪便等不同样本的特点采用相适合的DNA提取方法,并对肌肉、皮张、毛发、骨骼的提取进行了改进,通过对线粒体细胞色素b和控制区基因PCR扩增反应以及测序结果证实,这几种DNA抽提方法及相应改进的可靠性,并可以提高野外非损伤性取材在保护遗传学中的应用。
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| 关 键 词： | 獐 DNA提取 保护遗传学 |
| 文章编号： | 1000-7083(2006)03-0481-04 |
| 修稿时间： | 2005年10月21 |
Optimum Methods for DNA Extraction from Different Parts of Chinese Water Deer |
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| CHEN Min,ZHANG En-di.Optimum Methods for DNA Extraction from Different Parts of Chinese Water Deer[J].Sichuan Journal of Zoology,2006,25(3):481-484. |
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| Authors: | CHEN Min ZHANG En-di |
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| Abstract: | Muscle,fur,blood spots,bone,and feces of Chinese water deer for genetic research were collected.Several DNA extraction methods were improved and applied to muscle,fur,blood spots,and bone.Mitochondrial DNA cytochrome b and D-loop gene were amplified and sequenced successfully from those DNA,suggesting that the methods of DNA extraction were reliable,efficient and with extend value to noninvasive samples in conservation genetics study. |
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| Keywords: | Chinese water deer DNA extraction conservation genetics |
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