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我国32种鸟类DNA条形码分析
引用本文:马明义,闫颖,王译伟,李静,蔡延森,李佳凌.我国32种鸟类DNA条形码分析[J].四川动物,2012,31(5):729-733.
作者姓名:马明义  闫颖  王译伟  李静  蔡延森  李佳凌
作者单位:1. 泸州医学院,四川泸州,646000
2. 四川大学生命科学学院,四川省濒危野生动物保护生物学重点实验室,成都610064
摘    要:DNA条形码是一种分子分类方法,近年来在物种鉴定方面得到迅速的发展和应用.本研究分析了我国27属32种鸟类(61只)的线粒体细胞色素c氧化酶亚基Ⅰ(COⅠ)基因的条形码片段,分别用阈值法、聚类法和诊断核苷酸进行了分析,探究DNA条形码鉴定我国鸟类的准确性.结果显示,种内CO Ⅰ序列变异很小,种间存在较多的变异位点,种间的遗传距离显著大于种内的遗传距离,DNA条形码序列能够鉴定所有鸟类.

关 键 词:DNA条形码  COⅠ  动物  物种鉴定

A Study of DNA Barcoding on 32 Species of Bird in China
MA Ming-yi , YAN Ying , WANG Yi-wei , LI Jing , CAI Yan-sen , LI Jia-ling.A Study of DNA Barcoding on 32 Species of Bird in China[J].Sichuan Journal of Zoology,2012,31(5):729-733.
Authors:MA Ming-yi  YAN Ying  WANG Yi-wei  LI Jing  CAI Yan-sen  LI Jia-ling
Institution:1(1.Luzhou Medical College,Luzhou,Sichuan Province 646000,China;2.Key Laboratory of Conservation Biologyon Endangered Wildlife,Sichuan Province,College of Life Sciences,Sichuan University,Chengdu 610064,China)
Abstract:DNA barcoding has been proposed as a promising tool for the rapid species identification in a wide range of animal taxa.In this study,the ~648 bp mitochondrial cytochrome c oxidase subunit Ⅰ(COⅠ) gene of 61 individuals from 32 bird species was analyzed.By applying three methods(distance based method,monophyly-based method,and diagnostic characters),our result shows that COⅠ is able to distinguish all species studied.The present study validated the effectiveness of barcoding for the identification of birds.
Keywords:DNA barcode  COⅠ  animal  species identification
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