| 草鱼生长激素基因在毕赤酵母中的表达 |
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| 引用本文： | 孟亚鹏,徐敏,代焕梅,丁秀琼,张海英,殷雪,刘世贵,龙章富.草鱼生长激素基因在毕赤酵母中的表达[J].四川动物,2006,25(1):68-71. |
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| 作者姓名： | 孟亚鹏 徐敏 代焕梅 丁秀琼 张海英 殷雪 刘世贵 龙章富 |
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| 作者单位： | 1. 四川大学生命科学学院,成都,610064
2. 四川大学生命科学学院,成都,610064;成都川大创新生物技术研究所 |
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| 基金项目： | 全国教育科学规划项目;四川省科研项目;四川省应用基础研究计划 |
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| 摘 要： | 将草鱼生长激素基因（cGH）的cDNA亚克隆到酵母表达载体pPIC9K中，经电击转化导入毕赤巴斯德酵母GS115菌株，获得转化子。菌落PCR技术筛选证实cGH已经整合到了酵母染色体上。对重组酵母进行诱导表达，SDS-PAGE和Western印迹分析，结果表明cGH的毕赤巴斯德酵母GS115菌株中获得了高效表达。
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| 关 键 词： | 草鱼 生长激素基因 pPIC9K表达载体 毕赤酵母 高效表达 |
| 文章编号： | 1000-7083(2006)01-0068-04 |
| 收稿时间： | 2005-06-03 |
| 修稿时间： | 2005-07-01 |
Expression of Grass Carp Growth Hormone in Pichia pastoris |
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| MENG Ya-peng,XU Min,DAI Huan-mei,DING Xiu-qiong,ZHANG Hai-ying,YIN Xue,LIU Shi-gui,LONG Zhang-fu.Expression of Grass Carp Growth Hormone in Pichia pastoris[J].Sichuan Journal of Zoology,2006,25(1):68-71. |
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| Authors: | MENG Ya-peng XU Min DAI Huan-mei DING Xiu-qiong ZHANG Hai-ying YIN Xue LIU Shi-gui LONG Zhang-fu |
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| Institution: | 1. College of Life Sciences, Sichuan University, Chengdu 610064; 2. Institute of Chengdu Chuangxin Biotechnology, Sichuan University |
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| Abstract: | The ORF of grass carp growth hormone(cGH) was isolated and cloned from pGEX4T-1-cGH plasmid and inserted into pPIC9K,expression vector.The Pichia pastoris yeasts strain GS115 were transformed by electrophoration with pPIC9K-cGH and were screened on medium with G418.Colony PCR results showed that CGH gene had been integrated into the yeast chromosomes.By analyses of the SDS-PAGE and Western blotting,it was showed that cGH protein was expressed in Pichia pastoris GS115. |
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| Keywords: | grass carp growth hormone gene Pichia pastoris yeast expression |
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