| 当归对宫内缺氧新生大鼠大脑皮质神经元与VEGF mRNA表达的影响 |
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| 引用本文： | 梅欣明,刘敦玉,赵峰,马晶,余鸿.当归对宫内缺氧新生大鼠大脑皮质神经元与VEGF mRNA表达的影响[J].四川动物,2010,29(1). |
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| 作者姓名： | 梅欣明 刘敦玉 赵峰 马晶 余鸿 |
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| 作者单位： | 1. 泸州医学院组胚教研室,四川泸州,646000
2. 泸州医学院附属中医院急诊科,四川泸州,646000 |
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| 基金项目： | 四川省科技厅课题，四川省教育厅课题 |
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| 摘 要： | 目的 探讨宫内缺氧对新生大鼠大脑皮质神经元与VEGF mRNA表达的影响以及当归的调控作用.方法 孕14 d健康SD雌性大鼠15只,随机分为对照组、缺氧组和当归组各5只,于孕14 d开始将当归组与缺氧组孕鼠置于低张氧浓度三气培养箱中,制作胎鼠宫内缺氧模型,此前一小时按8 mL/kg分别给予当归和生理盐水尾静脉注射,对照组不缺氧,余同缺氧组.三组孕鼠分娩当日每窝随机选取新生鼠4只,取脑组织多聚甲醛固定,石蜡包埋切片、NSE mRNA、VEGF mRNA原位杂交,400倍拍照、IPP6.0软件图像分析.结果 缺氧组新生大鼠大脑皮质NSE mRNA阳性细胞数较对照组减少,积分光密度值(IOD)降低(P＜0.05),VEGF mRNA阳性细胞IOD值升高(P＜0.05);当归组新生大鼠大脑皮质NSE mRNA阳性细胞数较缺氧组增多、IOD值增高(P＜0.05),VEGF mRNA阳性细胞IOD值增高(P＜0.05).结论 宫内缺氧可致新生大鼠大脑皮质神经元受损,当归注射液对此损伤有一定保护作用,其机制可能是通过上调大脑皮质VEGF mRNA的表达而使缺氧环境改善.
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| 关 键 词： | 皮质 神经元 当归 缺氧 |
Effect of Angelica on Neurons and the Expression of VEGF mRNA in the Cerebral Cortex of Neonatal Rats Following Intrauterine Hypoxia |
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| MEI Xin-ming,LIU Dun-yu,ZHAO Feng,MA Jing,YU Hong.Effect of Angelica on Neurons and the Expression of VEGF mRNA in the Cerebral Cortex of Neonatal Rats Following Intrauterine Hypoxia[J].Sichuan Journal of Zoology,2010,29(1). |
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| Authors: | MEI Xin-ming LIU Dun-yu ZHAO Feng MA Jing YU Hong |
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| Institution: | MEI Xin-ming1,LIU Dun-yu2,ZHAO Feng1,MA Jing1,YU Hong1(1.Department of Histology , Embryology,Luzhou Medical College,Luzhou,Sichuan Province 646000,China,2.Department of Emergency,Traditional Chinese Medicine Hospital Affiliated to Luzhou Medical College,China) |
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| Abstract: | Objective To explore the effect of intrauterine hypoxia on the neurons and the expression of VEGF mRNA in the cerebral cortex of neonatal rats,and the regulative role of angelica to them.Method Fifteen pregnant healthy SD rats were divided randomly into three groups:a control group(CG),a hypoxia group (HG) and an angelica group (AG),with each group containing 5 rats.The angelica group and hypoxia group pregnant rats were placed in a three-gas incubator from the start of pregnant 14th days after to produce t... |
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| Keywords: | VEGF cortex neurons VEGF angelica hypoxia |
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