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| 应用40对微卫星引物对6种近交系小鼠遗传背景进行监测的研究 | |
| 引用本文： | 李军林,魏泓,张耀光,张树辉.应用40对微卫星引物对6种近交系小鼠遗传背景进行监测的研究[J].四川动物,2001,20(3):119-122. |
| 作者姓名： | 李军林 魏泓 张耀光 张树辉 |
| 作者单位： | 1. 第四军医大学生物学教研室, 2. 第三军医大学医学实验动物中心 3. 西南师范大学生命科学学院 |
| 基金项目： | 国家自然科学基金(39600079)、全军九五医药卫生科研基金(96M038)及重庆市攻关资助项目。 |
| 摘 要： | 采用40对引物的微卫星DNA PCR遗传质量符合要求的BALB／C－nu0nu-,DBA/2,SCID,T739,TA2,615等6种近交系小鼠进行遗传监测，结果26对引物有稳定的扩增结果，5对引物表现为单态性，21对引物表现出多态性，其中D2Nds3,D3Mitl5,D3Mitl7,D3Mit18,D16Mit7等6对引物表现出显著的多态性，反映了各品系小鼠独特的遗传背景，可应用于区别小鼠品系，监测系间的遗传污染，为有关小鼠品系积累了遗传背景资料，有助于将实验动物的遗传监测从表墼这度到DNA水平。 |
| 关 键 词： | 近交系小鼠 微卫星DNA多态性 遗传监测 |
| 文章编号： | 1000-7083(2001)03-0119-04 |
| 修稿时间： | 2000年10月23 |
GENETIC MONITORING OF SIX STRAINS OF INBRED MICE BY MICROSATELLITE DNA PCR WITH 40 PAIRS OF PRIMERS |
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| LI Jun lin ,WEI Hong ,ZHANG Yao gang ,ZHANG Shu hui.GENETIC MONITORING OF SIX STRAINS OF INBRED MICE BY MICROSATELLITE DNA PCR WITH 40 PAIRS OF PRIMERS[J].Sichuan Journal of Zoology,2001,20(3):119-122. | |
| Authors: | LI Jun lin WEI Hong ZHANG Yao gang ZHANG Shu hui |
| Institution: | LI Jun lin 1,WEI Hong 2,ZHANG Yao gang 3,ZHANG Shu hui 2 |
| Abstract: | Six strains of inbred mice including BALB/c nu nu,DBA/2,SCID,T 739 ,TA 2,615 were checked by microsatellite DNA PCR with 40 pairs of primers.The results showed that 26 pairs of primers had stable affection,of which 21 pairs of primers existed diversity in their amplified products and 5 showed monomorphic.The 6 such as D2Nds3,D3Mit15,D3Mit17,D3Mit18,D11Mit2 and D16Mit7 had more obvious polymorphism,which could be used for strains identification and genetic monitoring.The research proved that microsatellite DNA PCR was suitable for genetic monitoring of laboratory mice.It would be helpful for genetic monitoring of laboratory animal from phenotype to DNA. |
| Keywords: | inbred mouse microsatellite DNA polymorphism genetic monitoring |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |