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Effects of photoperiod,salinity and pH on cell growth and lipid content of Pavlova lutheri |
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| Authors: | Syed Muhammad Usman Shah Che Che Radziah Saadiah Ibrahim Faazaz Latiff Mohd Fariduddin Othman Mohd Azmuddin Abdullah |
| Institution: | 1. Department of Chemical Engineering, Universiti Teknologi PETRONAS, 31750, Bandar Seri Iskandar, Perak, Malaysia 2. Centre of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia 3. Fisheries Research Institute, 11960, Batu Maung, Pulau Pinang, Malaysia 4. Fisheries Research Institute, Pulau Sayak, 08500, Kota Kuala Muda, Kedah, Malaysia |
| Abstract: | The aim of the present work was to study the effects of photoperiod, salinity and pH on growth and lipid content of Pavlova lutheri microalgae for biodiesel production in small-scale and large-scale open-pond tanks. In a 250-mL flask, the cultures grew well under 24 h illumination with maximum specific growth rate, μ max , of 0.12 day?1 and lipid content of 35 % as compared to 0.1 day?1 and 15 % lipid content in the dark. The salinity was optimum for the cell growth at 30–35 ppt, but the lipid content of 34–36 % was higher at 35–40 ppt. Algal growth and lipid accumulation was optimum at pH 8–9. Large-scale cultivation in 5-L and 30-L tanks achieved μ max of 0.13–0.14 day?1 as compared to 0.12 day?1 in small-scale and 300L cultures. |
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