Use of Galleria mellonella larvae to evaluate the in vivo anti-fungal activity of [Ag2(mal)(phen)3] |
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| Authors: | Raymond Rowan Ciara Moran Malachy McCann Kevin Kavanagh |
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| Institution: | (1) Medical Mycology Unit, NICB, Department of Biology, National University of Ireland Maynooth, Co., Kildare, Ireland;(2) Department of Chemistry, National University of Ireland Maynooth, Co., Kildare, Ireland |
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| Abstract: | Larvae of the insect Galleria mellonella were employed to assess the in vivo antifungal efficacy of (Ag2(mal)(phen)3]), AgNO3 and 1,10-phenanthroline. Larvae pre-inoculated with these compounds were protected from a subsequent lethal infection by
the yeast Candida albicans while larvae inoculated 1 and 4 h post-infection showed significantly increased survival (P < 0.01) compared to control larvae. Administration of these compounds resulted in an increase over 48 h in the density of
insect haemocytes (immune cells) but there was no widespread activation of genes for antimicrobial peptides. This work demonstrates
that G. mellonella larvae may be employed to ascertain the antifungal efficacy of silver(I) compounds and offers a rapid and effective means
of assessing the in vivo activity of inorganic antimicrobial compounds. |
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| Keywords: | Antifungal Candida albicans Silver(I) Insect Galleria |
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