Rapid determination of bovine lactoferrin in dairy products by an automated quantitative agglutination assay based on latex beads coated with F(ab′)2 fragments |
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Authors: | K Yamauchi T Soejima Y Ohara M Kuga E Nagao K Kagi Y Tamura K Kanbara M Fujisawa S Namba |
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Institution: | Nutritional Science Laboratory, Zama, Kanagawa 228-8385, Japan. |
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Abstract: | This study evaluated an automated immunoassay for bovine lactoferrin (LF) in dairy products based on latex beads coated with F(ab')2 fragments. Methods: F(ab')2 fragments were obtained by pepsin digestion of rabbit anti-bovine LF (IgG fraction) and polystyrene latex beads were coated with the F(ab')2 fragments. We used the beads to develop a rapid and homogeneous light scatter immunoassay employing an autoanalyzer (the Automated Latex assay). The Automated Latex assay was easy to perform and could rapidly determine bovine lactoferrin in lactoferrin-supplemented products. It was sensitive enough for testing products and showed good precision. |
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Keywords: | automated bovine lactoferrin latex assay |
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