Pancreatic islets of variable size--insulin secretion and glucose utilization |
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Authors: | R M Colella S Bonner-Weir L P Braunstein M Schwalke G C Weir |
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Institution: | From the Department of Internal Medicine Medical College of Virginia Richmond, Virginia 23298, USA |
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Abstract: | Glucose metabolism and insulin secretion were studied in isolated rat pancreatic islets of different sizes and the amount of tissue was quantitated by the measurement of DNA. It was found that larger islets (140-210 ng DNA/islet) utilized more glucose (based on the conversion of 3H-5-glucose to 3H]20) per ng of DNA than islets containing less DNA (60-120 ng/islet). However, the insulin secreted per ng of DNA in response to a given glucose concentration was the same in islets of all sizes. Also, the islet insulin and glucagon content when expressed in terms of DNA did not depend upon islet size. Thus, although glucose utilization rates expressed as a function of islet DNA content were greater in larger islets, no such relationship was found for glucose-induced insulin release or insulin and glucagon content. |
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Keywords: | Address Correspondence to: G C Weir M D Joslin Diabetes Center One Joslin Place Boston MA 02215 USA |
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