| 蛋白激酶C参与缺氧预处理的血管平滑肌细胞保护 |
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| 引用本文： | 刘秀华,武旭东.蛋白激酶C参与缺氧预处理的血管平滑肌细胞保护[J].中国应用生理学杂志,1997,13(3):213-215. |
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| 作者姓名： | 刘秀华 武旭东 |
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| 作者单位： | 北京医科大学心血管基础研究所 |
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| 摘 要： | 已知缺氧预处理不仅对心肌细胞，而且对血管床亦有保护作用；但对血管壁细胞是否有直接保护作用，目前尚不清楚。本工作在培养的家兔血管平滑肌细胞（ＶＳＭＣ）缺氧复氧（Ａ／Ｒ）损伤模型上观察缺氧预处理（ａｎｏｘｉｃｐｒｅｃｏｎｄｉｔｉｏｎｉｎｇ，ＡＰＣ）的影响。发现ＡＰＣ能提高Ａ／Ｒ后ＶＳＭＣ存活率，减轻细胞脂质过氧化损伤和钙超载，使用蛋白激酶Ｃ（ＰＫＣ）激动剂ＰＭＡ能模拟，而抑制剂Ｈ７或ｐｏｌｙｍｙｘｉｎＢ能完全消除ＡＰＣ的上述保护作用。提示ＡＰＣ对ＶＳＭＣ的Ａ／Ｒ损伤具有保护作用，其机理可能与ＰＫＣ激活有关
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| 关 键 词： | 预处理 蛋白激酶C 血管平滑肌细胞 |
PROTEIN KINASE C INVOLVED IN THE PROTECTTVE EFFECT OF ANOXIC PRECONDITIONING ON VASCULAR SMOOTH MUSCLE CELLS |
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| Liu Xiuhua,Wu Xudong,Wang Shuheng,Pang Yongzheng,Su Jingyi,Tang Chaoshu.PROTEIN KINASE C INVOLVED IN THE PROTECTTVE EFFECT OF ANOXIC PRECONDITIONING ON VASCULAR SMOOTH MUSCLE CELLS[J].Chinese Journal of Applied Physiology,1997,13(3):213-215. |
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| Authors: | Liu Xiuhua Wu Xudong Wang Shuheng Pang Yongzheng Su Jingyi Tang Chaoshu |
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| Institution: | Institute of Cardiovascular Research, Beijing Medical University. |
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| Abstract: | It has been known that anoxic preconditioning (APC) has protective effects not only on cardiomyocyte, but also on vasculature. However , the effects of APC on vascular smooth muscle cells (VSMC) is unknown . On the model of anoxia/reoxygenation (A/R) injury of rabbit VSMC, the effect of APC was observed . It was found that APC could increase the survival rate of VSMC after A/R injury , lower the leakage of lactate dehydrogenase (LDH) and protein from cells, and attenuate peroxidation injury and calcium overload of VSMC. The results also showed that PMA, an activator of protein kinase C (PKC), could mimic , but H 7 or polymyxin B ( inhibitors of PKC) could abolish the above protective effect of APC. It is suggested that the protective effect of APC on VSMC during A/R injury might be due to the activation of PKC. |
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| Keywords: | preconditioning protein Kinase C vascular smooth muscle cells |
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