缺氧时大鼠红细胞变形性损伤的机制研究

本实验通过测定平原和模拟高原减压缺氧３０天大鼠红细胞滤过指数（ＩＦ）、红细胞内［ｐＨ］ｉ、［Ｋ＋］ｉ／［Ｎａ＋］ｉ比值、［Ｃａ２＋］ｉ、［Ｍｇ２＋］ｉ、平均红细胞体积（ＭＣＶ）及平均红细胞血红蛋白浓度（ＭＣＨＣ），从而探讨缺氧条件下大鼠红细胞变形性损害的机制。结果发现：１．缺氧组大鼠红细胞［Ｃａ２＋］ｉ明显升高，且与ＩＦ呈显著正相关，但［Ｍｇ２＋］ｉ无明显差异；２．缺氧组［Ｋ＋］ｉ／［Ｎａ＋］ｉ值较平原组明显降低，且与ＩＦ呈显著负相关；３．缺氧组ＭＣＨＣ与平原组无明显差异，但ＭＣＶ显著升高；４．缺氧组红细胞内［ｐＨ］ｉ较平原组明显升高。提示：缺氧时红细胞［Ｃａ２＋］ｉ升高，［Ｋ＋］ｉ／［Ｎａ＋］ｉ值降低，ＭＣＶ增大以及红细胞［ｐＨ］ｉ值的改变在其变形性损伤中起重要作用。

STUDIES OF MECHANISM OF ERYTHROCYTE DEFORMABILITY INJURY DURING HYPOBARIC HYPOXIA IN RATS

Rats were randomized into sea level group and hyoxic group. Erythrocyte filtration index (IF), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), erythrocyte pH]i, Mg2-]i, Ca2+]i and K+]i/Na+]i were investigated in our studies. The results were as follows: 1. IF was increared in hypoxic group (0.234 +/- 0.060) 2. Ca2+]i of hypoxic group (7.02 +/- 1.66) was evidently increased and correlated with its IF (r = 0.7386, P < 0.01), but Mg2+]i had no obvious changes, K+]i/ Na+]i ratio of hypoxic group was reduced and negatively correlated with IF (r = -0.3957, P < 0.05), meanwhile, pH] of hypoxic group increased evidently. 3. There was no statistic difference between MCHC of hypoxic and sea level group, but MCV of hypoxic group increased. These findings suggest that the changes of Ca2+]i, K+]i/Na+]i ratio, pH]i and MCV may play important role in erythrocyte deformability injury during hypoxia.