力竭运动及恢复期大鼠纹状体5-HT、DA及其代谢物浓度的动态变化研究

目的：观察一次性力竭运动过程及恢复期大鼠纹状体细胞外液中多巴胺（DA）和5-羟色胺（5-HT）及其代谢物浓度的动态变化规律。方法：采用活体微透析结合毛细管电泳．激光诱导技术,连续观察清醒大鼠在一次性力竭运动过程及恢复期纹状体细胞外液中酪氨酸（Tyr）、5-HT、5-羟吲哚乙酸（5-HIAA）、色氨酸（Trp）和DA浓度的动态变化。结果：大鼠纹状体细胞外液中TW、5-HT、5-HIAA水平运动初期均未见显著变化（P〉0．05）,运动后期、力竭及恢复期均显著高于安静水平（P〈0．05,P〈0．01）;DA、Tyr水平在运动后期、力竭及恢复期显著高于安静水平（P〈0．05,P〈0．01）;DA／5-HT运动初期显著升高（P〈0．05,P〈0．01）,运动后期出现下降趋势,力竭前15min降至最低点。而恢复期略有回升,但运动后期、力竭及恢复期与安静状态相比均无显著差异。结论：力竭运动过程中大鼠纹状体细胞外液中DA和5-HT的动态变化具有阶段性特征,运动疲劳过程中状体内DA和5-HT两种神经递质的代谢水平均显著增强,而其中以5-HT的作用占优。

Dynamic changes of 5-HT, DA and their metabolin in rat striatum during exhaustive exercise and recovery

Objective： To study the dynamic characteristics of serotonin （5-HT）, dopamine （DA） and their metabolin changes in brain during the development of exercise-induced central fatigue. Methods： Coupling of microdialysis and capillary electrophoresis-laser induced fluorescence detection method were used to continuously monitored the changes of DA, tryptophan （Trp）, tyrosine （Tyr）, 5-HT and 5-hydroxyindoleacetic acid （5-HIAA） in striatum extracellular fluid during the exhaustive exercise and recovery time. Results： The concentrations of Trp, 5-HT, 5-HIAA in striatum extracellular fluid had no remarkable changes in the early time of exercise（ P 〈 0.05）, while they significantly increased during the later time of exercise and whole recovery time （ P 〈0.05, P 〈 0.01）. The concentrations of DA and Tyr significantly increased over basal level in the later exercise time, exhaust and recovery time （ P 〈 0.05, P 〈 0.01）. DA/5-HT signifcanfly increased in the initial time of exercise （ P 〈 0.05, P 〈 0.01）, while decreased during the later exercise time, the nadir occurred at 15 minutes before rats exhausted. DA/5-HT slightly recovered back to basal level during the recovery time, and there was no significant difference during later exercise, exhausted and recovery time compared with basal level（ P 〈0.05）. Conclusion： The changes of DA and 5-HT in striatuln have phase characteristics. Both of them significantly increase during the development of exercise-induced fatigue. However, the 5-HT plays the dominant role in the dynamic changes of them.