肺叶切除术对红细胞及淋巴细胞膜流动性的影响

目的：研究肺叶切除术对红细胞及淋巴细胞膜流坳性的影响。方法：选择２０例择期开胸手术病人，均作肺叶切除术。分别用微量滴定法、高效液权色谱法和ＤＰＨ荧光探剂法测定血浆和红细胞膜ＰＬＡ２活性，红细胞膜磷脂ＰＳ、ＰＥ、ＰＣ和红细胞、淋巴膜脂流动性。结果：手术１０ｍｉｎ、手术６０ｍｉｎ和手术结束后３０ｍｉｎ血浆和红细胞膜ＰＬＡ２活性均显著高于麻醉诱导前；手术６０ｍｉｎ和手术结束后３０ｍｉｎ 红细胞膜ＰＳ、Ｐ

THE EFFECTS OF PULMONARY LOBECTOMY ON THE MEMBRANE FLUIDITY OF RBC AND LYMPHOCYTE

Aim:To investigate the effects of pulmonary lobectomy on the membrane fluidity of RBC and lymphocytes. Methods:Twenty patients undergoing pulmonary lobectomy were studied to examine the alteration of the membrane fluidity of RBC and lymphocytes during operative period.The PLA 2 activity, phosphatidylserine (PS),phosphatidylethanolamine(PE), phosphatidylcholine (PC) contents and membrane fluidity of RBC and lymphocyte were analysed by high performance liquid chromatograph and DPH method respectively.Results:PLA 2 activity in plasma and membrane of RBC was increased significantly at operative 10 min,60 min and postoperative 30 min compared with control (before induction of anesthesia). The PS, PE and PC content in membrane of RBC reduced significantly at operative 60 min and postoperative 30 min compared with control.The membrane fulidity of RBC and lymphocyte did not change significantly,but decreased significantly at operative 60 min and postoperative 30 min.Conclusion:Pulmonary lobectomy increased PLA 2 acticity, reduced the contents of phospolipid in cell membrane, and decreased membrane fluidity of RBC and lymphocyte.