| 秀丽线虫物理低氧损伤模型的建立与应用 |
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| 作者姓名： | Ren CH Zhang JY Shi JP Jiang B Zhao N Liu HQ Zhang CG |
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| 作者单位： | 军事医学科学院放射与辐射医学研究所蛋白质组学国家重点实验室医学神经生物学国家重点实验室;西北农林科技大学生命科学学院; |
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| 基金项目： | 国家973计划项目(2006CB504100); 国家科技重大专项课题(2009ZX09103-616,2009ZX09301-002,2009ZX09503-002); 国家自然科学基金资助项目(30900862,30771230); 医学神经生物学国家重点实验室开放基金课题(09-03); 中国博士后科学基金特别资助基金(200801495);中国博士后科学基金(20060400051) |
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| 摘 要： | 目的:利用秀丽线虫研发合适的低氧损伤模型,以更好地揭示低氧生理和低氧病理的分子机制.方法:通过对秀丽线虫进行不同时间的低氧处理,系统观察线虫的死亡率、运动功能、细胞形态及相关蛋白表达水平的变化,分析低氧对线虫的损伤情况.结果:氧浓度为0.2%的物理性低氧可引起秀丽线虫多种细胞形态发生变化,进而导致线虫死亡,且死亡率随低...
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| 关 键 词： | 秀丽线虫 物理低氧 转塞因线虫 物理低氧模型 |
Development and application of the physical hypoxic models of C. elegans |
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| Ren CH,Zhang JY,Shi JP,Jiang B,Zhao N,Liu HQ,Zhang CG.Development and application of the physical hypoxic models of C. elegans[J].Chinese Journal of Applied Physiology,2011,27(3):257-262. |
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| Authors: | Ren Chang-hong Zhang Ji-ye Shi Jin-ping Jiang Bin Zhao Na Liu Hu-qi Zhang Cheng-gang |
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| Institution: | REN Chang-hong1,ZHANG Ji-ye1,2,SHI Jin-ping1,JIANG bin1,ZHAO Na2,LIU Hu-qi1,ZHANG Cheng-gang1,2(1.Beijing Institute of Radiation Medicine,State Key Laboratory of Proteomics,State Key Laboratory of Medical Neurobiology,Beijing 100850,China,2.Department of Biology College of Life Science,Northwest Agriculture and Forestry University,Shaanxi 712100,China) |
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| Abstract: | Objective:To develop a suitable hypoxic injury model,which is important for revealing pathological molecular mechanism of hypoxia.Methods: We focused on C.elegans by treatment with different hypoxic times and systematically observed mortality,movement,cellular morphology and the related-protein expression of the animals.Results: We demonstrated that hypoxia(0.2% partial pressure of oxygen) induced morphological cell defects,and then leading to death of C.elegans.The mortality of C.elegans increased along wi... |
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| Keywords: | Caenorhabditis elegans physical hypoxia transgenic worm physical hypoxic model |
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