AGS3蛋白对吗啡慢性处理大鼠前额叶皮层神经元瞬时外向钾电流的影响

目的:研究吗啡对大鼠皮层神经元瞬时外向钾电流(IA)的影响,并在此基础上,用G蛋白信号转导激活因子3(AGS3)的抗体阻断AGS3作用,观察其对瞬时外向钾电流(IA)的影响,从而探讨AGS3蛋白在吗啡成瘾中的机制。方法:采用全细胞膜片钳技术记录IA;吗啡对神经元IA电流密度-电压曲线(I-V曲线)的影响;在全细胞构型下,观察三种不同浓度AGS3抗体对吗啡处理大鼠前额叶皮层神经元IA的影响。结果:吗啡能引起大鼠皮质神经元IA增强;当膜电位+55mV时,10-3μg/L、10-2μg/L、10-1μg/L三种不同浓度的AGS3抗体作用于吗啡处理的神经元,10-3μg/L对电流密度的抑制没有显著差异;10-2μg/L、10-1μg/L的抗体能显著抑制吗啡引起的电流密度升高,有统计学差异。结论:吗啡能引起神经元I的增强,AGS3蛋白在成瘾机体中参与了对I通道进行调节的信号转导通路。

The effect of AGS3 on the I_A of newborn rat prefrontal cortical neurons pretreated by chronic morphine

Objective：The effect of chronic morphine treatment on the IA （transient outward K＋ current） of prefrontal cortical neurons of newborn rat.On this basis,we use AGS3 antibody to inhibit the function of AGS3,for observing the impact of AGS3 on the IA,thus further explore the mechanism of AGS3 protein in morphine addiction.Methods：By using whole-cell patch-clamp technique,IA was recorded.In the whole-cell configuration,observed the impact of morphine on the current density-voltage curve（I-V）of IA and the effect of AGS3 antibody with three different concentrations on the IA of morphine treated rat prefrontal cortical neurons.Results：Morphine increased the IA.When the test potential was ＋55 mV,different concentrations of AGS3,10-3 μg/L,10-2 μg/L and 10-1 μg/L acted on morphine treated rat prefrontal cortical neurons,the enhanced IA by morphine was inhibited.Conclusion：Morphine increases the IA,AGS3 protein may participate in signal transduction pathway involved with IA.