NADPH氧化酶抑制剂apocynin对力竭运动大鼠运动性蛋白尿的影响

目的：研究NADPH氧化酶抑制剂apocynin对力竭运动大鼠运动性蛋白尿产生的影响及其机制。方法：32只SD雄性大鼠随机分为安静对照组（C组）、对照+药物组（CA组）、力竭运动组（E组）、力竭运动+药物组（EA组）。药物注射按10 mg/kg体重，每天一次，连续3 d，并在末次药物注射1 h后进行一次性跑台力竭运动。测定运动后尿UP、血液BUN水平、肾脏ROS浓度、NOS活性、NOS与3-NT蛋白含量。结果：结果显示，E组UP、肾脏ROS、iNOS含量及活性、3-NT明显升高，而EA组的这些指标与C组相比无显著性差异。结论：力竭运动可明显增加肾组织NADPH氧化酶活性，从而产生大量的ROS，后者可迅速地与由肾脏iNOS催化生成的NO反应，产生过量的ONOO^-，诱发运动性蛋白尿的生成。

Effect of NADPH oxidase inhibitor apocynin on the exercise induced proteinuria in rats

Objective: To investigate the effect of NADPH oxidase inhibitor apocynin on exercise-induced proteinuria and its related mechanism. Methods: Thirty-two SD rats were randomly divided into the control group (group C), control + drug group (group CA), exhaustive exercise group (group E), exhaustive exercise + drug group (group EA). The rats were administrated apocynin at 10 mg/kg weight,once a day for three days, and one hour after the drug injection, a one-time exhaustive exercise was performed. After exhaustive exercise, urine protein, blood urea nitrogen (BUN), and kidney reactive oxygen species (ROS) concentration, NOS activity, NOS and 3-NT concentration were detected. Results: In comparison to control group urinary protein (UP), ROS, inductible nitric oxide synthase (iNOS), 3-NT levels increased significantly in group E while those in group EA did not change. Conclusion: The elevated renal NADPH oxidase activity by exhaustive exercise induced ROS that can rapidly react with NO, and then produces excess peroxynitrite, which contributes to occurrence of exercise-induced proteinuria.