红花注射液对慢性低O2高CO2肺动脉高压的影响

目的：探讨红花注射液对大鼠在慢性低O2高CO2下肺动脉高压的抑制作用。方法：将SD大鼠分为对照组,慢性低O2高CO2组,慢性低O2高CO2＋红花注射液组。用电镜、放免等方法,观察各组大鼠肺动脉平均压、颈动脉平均压、肺细小动脉显微结构、血浆和肺匀浆TXB2及6-keto-PGF1a含量的变化。结果：①慢性低O2高CO2组mPAP比对照组显著增高,红花注射液组的mPAP比慢性低O2高CO2组显著降低,3组间mCAP比较差异无显著性。②慢性低O2高CO2组与对照组相比血浆和肺匀浆TXB2浓度、TXB2／6-keto-PGF1a比值显著增高,6-keto-PGF1a浓度显著下降;红花注射液组与慢性低P2高CO2相比血浆和肺匀浆TXB2浓度、TXB2／6-keto-PGF1a显著下降,6-keto-PGF1a显著升高。③光镜下慢性低O2高CO2组与对照组相比,肺细小动脉管壁面积／管总面积（WA／TA）和肺细小动脉中膜厚度（PAMT）均显著增高。红花注射液组WA／TA和PAMT显著降低。④电镜下慢性低O2高CO2组大鼠肺细小动脉内皮细胞吞饮小泡增多,血管壁增厚,中膜平滑肌细胞增生,纤维细胞增多,肺泡Ⅱ型上皮细胞微绒毛脱落;红花注射液组肺细小动脉中膜平滑肌细胞增生减轻,纤维细胞少,胶原纤维减少,肺泡Ⅱ型上皮细胞微绒毛丰富、结构清。结论：红花注射液有减轻慢性低O2高CO2性肺动脉高压和肺血管结构重建的作用,可能与抑制TXA2的合成,保护血管内皮细胞,使TXA2/PGI2比值降低有关.

Effect of safflower injection on pulmonary hypertension in rat during chronic hypoxia and hypercapnia

Aim： To study the effect of Safflower injection（a compond of Chinese Traditional medicine） on pulmonary hypertension in rat during chronic hypoxia and hypercapnia. Methods： Sprague-Dawley rats were randomly divided into normal control group（A）, hypoxic hypercapnic group（B）, hypoxic hypercapnia ＋ Safflower injection group（C）. The concentration of TXB2 and 6-keto-PGF1a in plasma and in lung ho- mogenate were detected by the radioimmunoassay. Results： （1）mPAP, weight ratio of right ventricle（RV） to left ventricle plus septum（LV ＋ S） were much higher in rats of hypoxic hypercapnic group than those of control group. Differences of mCAP among the three groups were not significant. （2）The concentration of TXB2 and the ratio of TXB2/6-keto-PGFla were significantly higher in rats of B group than those of A and C group. （3）The results examined by light microscopy showed that WA/TA（vessel wall area/total area）, SMC（the density of medial smooth muscle cell） and PAMT（ the thickness of medial smooth cell layer） were significantly higher in rats of B group than those of A and C group. （4） The results examined by electron microscopy showed proliferation of medial smooth muscle cells and collagen fibers of pulmonary arterioles in rats of B group, and Safflower injection could reverse the changes mentioned above. Conclusion： Safflower injection may inhibit hypoxic hy- percapnia pulmonary hypertension and pulmonary vessel remodeling by decreasing the ratio of TXB2/6-keto-PGF1a.