牛磺酸对肢体缺血／再灌注后肝损伤大鼠TNF-α、NF-κB表达的影响

目的：探讨给予牛磺酸预处理对肢体缺血／再灌注（limb ischemia-reperfusion，H／R）后大鼠肝脏损伤及，INn、NF—KB表达的影响及意义。方法：采用Wistar大鼠建立LI/R损伤模型，随机分为4组（n=10）：对照（C）组，缺血／再灌注（I／R）组，牛磺酸（T）组和牛磺酸＋缺血／再灌注（TR）组。比色法测定动物血浆ALT、AST、MDA，肝组织MDA、MPO、DNA裂解率和钙含量，放免法检测血浆及肝组织TNF-α水平；HE染色观察肝脏组织形态改变；免疫组化法观察NF-κB蛋白表达。结果：与C组比较，I／R和TR组各损伤性指标、TNF-α水平均升高，NF-κB蛋白表达增高（P〈0．01）；但TR组上述各项指标较I／R组显著降低。结论：牛磺酸预处理可减轻大鼠LI／R所致肝脏损伤，降低TNF-α、NF-κB表达。

Effects of taurine on TNF-alpha and NF-kappaB expression of liver injury after limbs ischemia/reperfusion in rats

Aim： To investigate the effect of pretreatment with taurine on liver injury changes and the change of tumor necrosis factor alpha and NF-κB expression following rats limbs ischemia/reperfusion. Methods： The model of limbs ischemia/reperfusion injury on rats was adopted in the experiment. Wistar rats were randomized into 4 groups（ n = 10） ： Control group, T group, I/R group and TR group. Levels of alanine amino- transferase（ALT）, aspartate aminotransferase（AST） and MDA in the plasma, MDA, MPO, calcium in liver tissues were measured by colorimetric method. The content of TNF-α in plasma and liver tissues was determined by radioimmunoassay. The morphologic changes were observed with HE staining. The expressions of NF-κBp65 in liver tissues were tested by immuno-histochemistry method. Results： It was found that against the courol group, the test values of ALT, AST, et al. and expressions of TNF-α, NF-κB increased in I/R group and TR group, but va lues of those in TR group were lower than in I/R group. Conclusion： Tamine can decrease the levels of TNF-α and NF-κB. It can mitigate the liver injury after limb ischemia/reperfusion injury in rats.