NO对大鼠睡眠-觉醒的调节

目的和方法：通过对大鼠侧脑室微量注射ＮＯＳ抑制剂Ｌ－ＮＡＭＥ及ＮＯ的前体Ｌ－精氨酸（Ｌ－Ａｒｇ）观察两种物质对大鼠睡眠－觉醒的影响。结果：注射１ｍｇ Ｌ－ＮＡＭＥ（５μＬ）后４ｈ觉醒（Ｗ）明显增加，尤以注射后第１￣２ｈ显著；４ｈ慢波睡眠（ＳＷＳ）明显减少，该效应同样以注射后第１￣２ｈ显著；异相睡眠（ＰＳ）无明显变化。小剂量Ｌ－ＮＡＭＥ（０．２ｍｇ，５μｌ）对大鼠的Ｗ、ＳＷＳ、ＰＳ无明显影响；同样方

THE REGULATION OF NO ON SLEEP-WAKEFULNESS IN RATS

AIM AND METHODS: The effects of an inhibitor of nitric oxide synthase L-NAME and a precursor of NO(L-arginine) on sleep-wakefulness in rats were examined. RESULTS: 1. The time of waking was increased(P < 0.01) and the time of SWS was decreased(P < 0.01) during the first two hours after injection intracerebroventricularly(i.c.v.) of L-NAME 1 mg, this effect was showed across the 4 h recording period(P < 0.05). The i.c.v. injection of L-NAME 0.2 mg had no significant effect. 2. The time of waking was su-ppressed during the third and fourth hours after i.c.v. injection of L-arginine 300 micrograms(P < 0.05). At the same time the increase of SWS was observed (P < 0.01). 3. There was no change on W, SWS, PS when L-NAME 1 mg was given after administration of L-arginine. CONCLUSION: The excitatory effect of L-NAME on wakefulness is mediated by inhibition of NOS. This effect could be antagonized by L-arginine. It suggests that NO is involved in regulation of sleep-wakefulness.