不同压力二氧化碳气腹对兔血液流变学和微循环的影响

目的：研究不同压力二氧化碳气腹对兔血液流变学和微循环的影响。方法：18只雌性健康实验兔按气腹压力随机均分为三组：气腹压0mmHg组（Ⅰ组）、气腹压10mmHg组（Ⅱ组）和气腹压15mmHg组（Ⅲ组）。每组兔均在不同的压力下接受气腹1h。在二氧化碳气腹前5min（T0）、气腹后30min（T1）、气腹后60min（T2）测定血液流变学指标。监测心率（HR）、平均动脉压（MAP）、耳廓微循环的血流量和血流速率并记录在相应时点的上述参数值。结果：气腹后30min、60min,Ⅱ组与Ⅰ组比较,HR、MAP、全血粘度、红细胞刚性指数和聚集指数显著增加（P〈0.05）,红细胞变形指数、耳廓微循环的血流量和血流速率显著下降（P〈0.05）,Ⅲ组与Ⅰ组比较,各参数变化更为显著（P〈0.01）。血浆粘度和红细胞压积气腹前后无明显变化。结论：二氧化碳气腹后血液流变性减弱;虽然HR、MAP增加,但微循环的血流量和血流速率下降。

Effects of different intraabdominal pressure of carbon dioxide pneumoperitoneum on hemorrheology and microcirculation in rabbits

Objective：To study effects of different intraabdominal pressure of carbon dioxide（CO2） pneumoperitoneum on hemorrheology and microcirculation in rabbits. Methods： Eighteen femal healthy rabbits weighing 2.2 kg to 3.5 kg were randomly divided into three groups equally based on pneumoperitoneum pressure： 0 mmHg group （groupⅠ）,10 mmHg group（groupⅡ） and 15 mmHg （groupⅢ）. Each group received 1 h pneumoperitoneum under different pressure. Blood samples were taken at 5 min before CO2 pneumoperitoneum, at 30 and 60 min after pneumoperitoneum for the measurements of indexes of hemorrheology. Hemodynamics including heart rate（HR）, mean arterial pressure（MAP） and the volume and velocity of the microcirculation of auricle were continuously monitored, such indexes were recorded at the related time. Results： Afer pneumoperitoneum at 30 and 60 min, compared with groupⅠ, HR, MAP, the whole blood viscosity, the aggregation and rigid indexes of RBC were significantly raised in groupⅡ（P0.05）, the deformobility indexes of RBC , the volume and velocity of the microcirculation were markedly decreased（P0.05）. Even more significant changes were observed in groupⅢ（P0.01）. The plasma viscosity and the hematocrit changed little. Conclusion： After CO2 pneumoperitoneum, hemorrheology is decreased; Although HR、MAP are raised, the volume and velocity of the microcirculation are decreased.