无巨核细胞条件下促血小板生成素对骨髓基质细胞纤维形成的影响

目的：观察无巨核细胞存在的条件下促血小板生成素能否刺激骨髓基质细胞纤维形成。方法：用改良Dexter培养法进行体外不同浓度促血小板生成素（TPO）作用下的基质细胞培养,在培养过程中检测基质细胞相对增殖指数,纤维连接蛋白、层粘素和Ⅳ型胶原的表达,以及Ⅲ型前胶原蛋白的合成。结果：TPO可刺激基质细胞增殖,相对增殖指数随TPO浓度增加而增强,但不随作用时间延长而增强;纤维连接素、层粘素和Ⅳ型胶原在对照组与实验组均有阳性表达,但实验组强于对照组,但阳性强度不随培养时间的延长而增强;标记的Ⅲ型前胶原蛋白平均荧光强度实验组高于对照组,差异明显,但这种作用的强弱与TPO浓度相关性不强。结论：无巨核细胞存在的条件下,TPO可直接刺激骨髓基质细胞产生细胞外基质和胶原,促进其纤维形成。

The effects of thrombopoietin on the fibrogenesis of bone marrow stromal cells in absence of megakaryocytes

Objective：In order to investigate whether or not thrombopoietin（TPO） could promote the fibrogenesis of bone marrow stromal cells in absence of megakaryocytes（MKs）.Methods：Improved dexter culture system with various TPO concentrations was used for ex vivo culture of bone marrow stromal cells.Relative proliferation index,the expressions of fibronectin,laminin and type Ⅳ collagen, and the systhesis of type Ⅲ procollagen were detected at different time points during culture process.Results：TPO stimulated the proliferation of bone marrow stromal cells.Relative proliferation index of the stromal cells increased with the TPO concentration increasing,and was not related to the exposure time.The expressions of fibronectin,laminin,and type Ⅳ collagen appeared stronger in the TPO groups than those in the control group.But the expressions of these molecules were not dependent upon the culture time.TPO could accelerate the synthesis of type Ⅲ procollagen in bone marrow stromal cells,and this acceleration was unrelated to the TPO concentration.Conclusion：These findings suggested that TPO could stimulate the stromal cells with a consequence of increased syntheses and secretions of the extracellular matrix and collagen in absence of MKs.In other words,TPO could promote the fibrogenesis of bone marrow stromal cells without the existence of MKs.